Unsteady Aerodynamic Performance of Model Wings at Low Reynolds Numbers

The synthesis of a comprehensive theory of force production in insect flight is hindered in part by the lack of precise knowledge of unsteady forces produced by wings. Data are especially sparse in the intermediate Reynolds number regime (10<Re<1000) appropriate for the flight of small insects. This paper attempts to fill this deficit by quantifying the time-dependence of aerodynamic forces for a simple yet important motion, rapid acceleration from rest to a constant velocity at a fixed angle of attack. The study couples the measurement of lift and drag on a two-dimensional model with simultaneous flow visualization. The results of these experiments are summarized below. 1. At angles of attack below 13.5°, there was virtually no evidence of a delay in the generation of lift, in contrast to similar studies made at higher Reynolds numbers. 2. At angles of attack above 13.5°, impulsive movement resulted in the production of a leading edge vortex that stayed attached to the wing for the first 2 chord lengths of travel, resulting in an 80 % increase in lift compared to the performance measured 5 chord lengths later. It is argued that this increase is due to the process of detached vortex lift, analogous to the method of force production in delta-wing aircraft. 3. As the initial leading edge vortex is shed from the wing, a second vortex of opposite vorticity develops from the trailing edge of the wing, correlating with a decrease in lift production. This pattern of alternating leading and trailing edge vortices generates a von Karman street, which is stable for at least 7.5 chord lengths of travel. 4. Throughout the first 7.5 chords of travel the model wing exhibits a broad lift plateau at angles of attack up to 54°, which is not significantly altered by the addition of wing camber or surface projections. 5. Taken together, these results indicate how the unsteady process of vortex generation at large angles of attack might contribute to the production of aerodynamic forces in insect flight. Because the fly wing typically moves only 2–4 chord lengths each half-stroke, the complex dynamic behavior of impulsively started wing profiles is more appropriate for models of insect flight than are steady-state approximations

The wake dynamics and flight forces of the fruit fly Drosophila melanogaster

We have used flow visualizations and instantaneous force measurements of tethered fruit flies (Drosophila melanogaster) to study the dynamics of force generation during flight. During each complete stroke cycle, the flies generate one single vortex loop consisting of vorticity shed during the downstroke and ventral flip. This gross pattern of wake structure in Drosophila is similar to those described for hovering birds and some other insects. The wake structure differed from those previously described, however, in that the vortex filaments shed during ventral stroke reversal did not fuse to complete a circular ring, but rather attached temporarily to the body to complete an inverted heart-shaped vortex loop. The attached ventral filaments of the loop subsequently slide along the length of the body and eventually fuse at the tip of the abdomen. We found no evidence for the shedding of wing-tip vorticity during the upstroke, and argue that this is due to an extreme form of the Wagner effect acting at that time. The flow visualizations predicted that maximum flight forces would be generated during the downstroke and ventral reversal, with little or no force generated during the upstroke. The instantaneous force measurements using laser-interferometry verified the periodic nature of force generation. Within each stroke cycle, there was one plateau of high force generation followed by a period of low force, which roughly correlated with the upstroke and downstroke periods. However, the fluctuations in force lagged behind their expected occurrence within the wing-stroke cycle by approximately 1 ms or one-fifth of the complete stroke cycle. This temporal discrepancy exceeds the range of expected inaccuracies and artifacts in the measurements, and we tentatively discuss the potential retarding effects within the underlying fluid mechanics

Fragmentation of a dioxolanyl radical via nonstatistical reaction dynamics: characterization of the vinyloxy radical by ns time-resolved laser flash photolysis

The photochemistry of two Barton esters, one derived from a dioxolane carboxylic acid and the other from pivalic acid, was investigated by product analysis and nanosecond laser flash photolysis (LFP). As expected, photolysis of the pivalate ester resulted in formation of the pyridine-2-thiyl and the t-butyl radical. Photolysis of the Barton ester of 2,2-dimethyl-1,3-dioxolane-4-carboxylic acid, on the other hand, revealed a complex multi-step fragmentation. In addition to the pyridine-2-thiyl and dioxolanyl radical, we gained evidence for the formation of the vinyloxy radical, CH2[double bond, length as m-dash]CHO˙. The latter was identified in the LFP by its π-complexes with benzene and diphenylether, its rapid quenching by electron-rich arenes and tri-n-butyl tin hydride, and its oxidative power in presence of trifluoroacetic acid as demonstrated by the oxidation of ferrocene to ferrocenium. Formation of CH2[double bond, length as m-dash]CHO˙ can be rationalized via fragmentation of the dioxolanyl radical. As the calculated barriers are too high for the reaction sequence to occur on the LFP time scale, we investigated the fragmentation of the photoexcited Barton ester via Born–Oppenheimer molecular dynamics simulations. In one trajectory, we could observe all reaction steps including ring opening of the dioxolanyl radical, suggesting that the excess energy gained in the ester cleavage and decarboxylation may lead to fragmentation of the hot dioxolanyl radical

The active control of wing rotation by Drosophila

This paper investigates the temporal control of a fast wing rotation in flies, the ventral flip, which occurs during the transition from downstroke to upstroke. Tethered flying Drosophila actively modulate the timing of these rapid supinations during yaw responses evoked by an oscillating visual stimulus. The time difference between the two wings is controlled such that the wing on the outside of a fictive turn rotates in advance of its contralateral partner. This modulation of ventral-flip timing between the two wings is strongly coupled with changes in wing-stroke amplitude. Typically, an increase in the stroke amplitude of one wing is correlated with an advance in the timing of the ventral flip of the same wing. However, flies do display a limited ability to control these two behaviors independently, as shown by flight records in which the correlation between ventral-flip timing and stroke amplitude transiently reverses. The control of ventral-flip timing may be part of an unsteady aerodynamic mechanism that enables the fly to alter the magnitude and direction of flight forces during turning maneuvers

Defining properties of neural crest-derived progenitor cells from the apex of human developing tooth

The connective tissue of the human tooth arises from cells that are derived from the cranial neural crest and, thus, are termed as "ectomesenchymal cells." Here, cells being located in a pad-like tissue adjacent to the apex of the developing tooth, which we designated the third molar pad, were separated by the microexplant technique. When outgrowing from the explant, dental neural crest-derived progenitor cells (dNC-PCs) adhered to plastic, proliferated steadily, and displayed a fibroblast-like morphology. At the mRNA level, dNC-PCs expressed neural crest marker genes like Sox9, Snail1, Snail2, Twist1, Msx2, and Dlx6. Cytofluorometric analysis indicated that cells were positive for CD49d (alpha4 integrin), CD56 (NCAM), and PDGFRalpha, while negative for CD31, CD34, CD45, and STRO-1. dNC-PCs could be differentiated into neurogenic, chondrogenic, and osteogenic lineages and were shown to produce bone matrix in athymic mice. These results demonstrate that human third molar pad possesses neural crest-derived cells that represent multipotent stem/progenitor cells. As a rather large amount of dNC-PCs could be obtained from each single third molar, cells may be used to regenerate a wide range of tissues within the craniofacial region of humans

The Influence of Learning Management System Components on Learners’ Motivation in a Large-Scale Social Learning Environment

Blended learning is becoming increasingly important in information systems research and education. One major drawback is to foster student’s motivation to actively participate in blended learning environments. There is still little understanding about the perceived value of incentives and components of learning management systems. Therefore, we investigated the perceived value of incentives. Furthermore, we analyze incentives’ and LMS components’ influence on learner motivation during a large-scale lecture. Based on the theoretical background of the ARCS model, we found that the perceived value of self-tests and forums have a substantial influence on learners’ motivation. In particular, the findings suggest that a clear communication of the components’ benefits is likely to positively influence learners’ attention, confidence, and perceived relevance. We also show that lecturers can influence perceptions of components by means of incentives. These results are valuable to develop LMS environments

Silicification and organic matter preservation in the Anisian Muschelkalk: Implications for the basin dynamics of the central European Muschelkalk Sea

Anisian Muschelkalk carbonates of the southern Germanic Basin containing silicified ooidal grainstone are interpreted as evidence of changing pH conditions triggered by increased bioproductivity (marine phytoplankton) and terrestrial input of plant debris during maximum flooding. Three distinct stages of calcite ooid replacement by silica were detected. Stage 1 reflects authigenic quartz development during the growth of the ooids, suggesting a change in the pH–temperature regime of the depositional environment. Stages 2 and 3 are found in silica-rich domains. The composition of silica-rich ooids shows significant Al2O3 and SrO but no FeO and MnO, indicating that late diagenetic alteration was minor. Silicified interparticle pore space is characterized by excellent preservation of marine prasinophytes; palynological slides show high abundance of terrestrial phytoclasts. The implications of our findings for basin dynamics reach from paleogeography to cyclostratigraphy and sequence stratigraphy, since changes in the seawater chemistry and sedimentary organic matter distribution reflect both the marine conditions as well as the hinterland. Basin interior changes might overprint the influence of the Tethys Ocean through the eastern and western gate areas. Stratigraphically, such changes might enhance marine flooding signals. Ongoing research needs to address the complex interaction between an intracratonic basin and an open-ocean system by comparing local and regional biotic and abiotic signals

Structure of the pheromone peptide of the Staphylococcus epidermidis agr system

AbstractThe agr quorum-sensing system is responsible for the regulation of several virulence factors in staphylococci, with an extracellular pheromone peptide as signalling molecule. By monitoring the biological activity of synthetic peptides, it could be demonstrated that the pheromone of the agr system in Staphylococcus epidermidis is an octapeptide containing a thiolester linkage between the central cysteine and the C-terminal carboxyl group. The peptide was active at nanomolar concentrations. The N-terminus of the peptide pheromone, which is encoded as part of a protein precursor, proved to be crucial for biological activity